Composite

Part:BBa_K2598052:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-02)


lacZ+bFMO+gusA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
    Illegal AgeI site found at 3879
    Illegal AgeI site found at 4209
    Illegal AgeI site found at 4513
    Illegal AgeI site found at 4536
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

bFMO and gusA are reversed to avoid leakage.


Source

The subparts are provided by Christopher A Voigt.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)